DNase I Activity Retained after Heat Inactivation in Standard Buffer
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منابع مشابه
DNase I activity retained after heat inactivation in standard buffer.
For the detection of RNA transcripts by RT-PCR, prior removal of genomic DNA must be performed. To remove genomic DNA, RNA is often prepared by DNase I digestion following phenol extraction. Recently, one-tube or onebuffer systems of RT-PCR were developed to prevent loss of RNA and to reduce the risk of contamination (2,6). In these methods, DNase I is added before RT. Taq DNA polymerase prepar...
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In many applications of RT-PCR, residual genomic DNA in total RNA preparations is amplified together with RNA, which results in false-positive data. One way to overcome this problem is to design primers that span a region containing one or more introns, thereby creating a diagnostic size difference between the amplification products originating from the RNA or the DNA. However, this method will...
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概要 航DNA抗 体はSLEに 最 も特異的な 自己抗体 であるが,そ の産生機序は不明である.抗 体の 産生に抗原処理機能が重要 であ る点に注 目し, SLEにDNA代 謝異常の存在を想定 した.そ の点を 証 明す るためにご血清DNase I 活性 とその易熱性抑制因子を測定 し, SLEの 活動性,抗DNA抗 体価 との関連 を検討 した.さ らに他の膠原病 な どの炎症性疾患 と比較 した. SLEのDNA分 解能は抗D NA抗 体価 が高い活動期に低 く,症 状 の軽快 とともにいつたん高 くな り,非 活動期 で再び低下 した. 長期 間続 いた 非 活動期 にもDNase抑 制因子 は高値を維持 した.活 動性PSS, RAで はDNase抑 制因 子は高かつ たが, DNA分 解能は活動性 と一定 の傾 向がな く,感 染症 などの炎症疾患では炎症時にの み抑制 ...
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Several reliable methods to produce transgenic animals utilize the male genome. After penetration into oocyte, sperm DNA undergoes dramatic conformational changes that could represent a great opportunity for exogenous DNA to be integrated in the zygote genome. Among the enzymes responsible for sperm remodeling, a nuclease could be involved. The presence of a DNase I in oocytes has not been much...
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In our work the antitumor and antimetastatic activities of RNase A and DNase I were studied using two murine models of pulmonary (Lewis lung carcinoma) and liver (hepatoma A-1) metastases. We found that intramuscular administration of RNase A at the dose range of 0.1-50 µ g/kg retarded the primary tumor growth by 20-40%, and this effect disappeared with the increase in RNase A dose over 0.5 mg/...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00291bm05